High-throughput sequencing technology is now an essential tool in many domains of biology. In addition to enabling large scale population genomics (e.g. 1000 genomes project), the digital, quantitative readout of sequencing reads allows genome-wide insights into chromatin biology, gene expression, translation, and other molecular features. This course will focus on introducing students to uses of high throughput sequencing, including but not limited to population resequencing of genomic data, RNA sequencing (RNAseq), chromatin immunoprecipitation followed by sequening (ChIPseq), chromatin conformation analysis (Hi-C), and many other types of data. Students will be taught basic scripting skills in Python, as well the use of freely available software packages for data analysis.